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KMID : 0985420090310020287
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Laboratory Medicine and Quality Assurance
2009 Volume.31 No. 2 p.287 ~ p.291
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Interpretation of Positive Result for Influenza A and Negative Result for Novel Influenza A/H1N1 in Reverse Transcriptase PCR for Novel Influenza A/H1N1
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Lee Young-Kyung
Song Won-Keun
Kim Han-Sung
Kang Hee-Jung
Kim Jae-Seok
Lee Kyu-Man
Kim Hyun-Soo
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Abstract
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Background: Real-time reverse transcriptase PCR (rRT-PCR) is widely used to detect novel influenza A/H1N1. We had observed several cases with positive result for influenza A and negative result for novel influenza A/H1N1 during a novel influenza A/H1N1 outbreak. The causes of those results were investigated in this study
Methods: A total of 913 cases tested with rRT-PCR for novel influenza A/H1N1 (Real-time Ready Influenza A/H1N1 Detection Set, Roche Diagnostics, Germany) during 25 August 2009 to 8 September 2009 was enrolled in this study. Cases showing positive result for influenza A (M gene) and negative result for novel influenza A/H1N1 (H1 gene) were tested with multiplex RT-PCR for seasonal influenza and novel influenza A/H1N1 (Seeplex FluA ACE Subtyping kit, Seegene, Korea), and the amplicons were directly sequenced.
Results: One hundred and eleven cases (12.2%) were positive for novel influenza A/H1N1. Twenty-seven cases (3.0%) were positive for influenza A, but negative for novel influenza A/H1N1. Subtypes of influenza A were determined in 25 cases by multiplex RT-PCR and nucleotides sequencing. One novel influenza A/H1N1, six seasonal influenza A/H1N1, three seasonal influenza A/H3N2, and 15 influenza A/H9N2 were detected.
Conclusions: Subtypes of influenza A were determined in most cases with positive result for influenza A and negative result for novel influenza A/H1N1. Several cases with seasonal influenza A were detected. Even if a nonepidemic period of seasonal influenza, tests for seasonal influenza A can help in the differential diagnosis of influenza.
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KEYWORD
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Novel influenza A/H1N1, Seasonal influenza A, Real-time RT-PCR
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